Cultures of Emiliania huxleyi

John Green
Plymouth Marine Laboratory
Citadel Hill
Plymouth PL1 2PB, UK.
Fax: +44 (0)1752 633102)
Email: JGR@wpo.nerc.ac.uk

As part of the EHUX project, a collection of some 300 strains of Emiliania huxleyi were isolated from a variety of marine habitats (coastal and oceanic) in different parts of the world, and have subsequently been maintained. These have been a source of material for experimental work on aspects of the physiology, genetics, biochemistry and ecology of this species and many of these strains are still available for distribution. For further information, please contact Dr. J.C. Green (address above).

Recent Isolates: N. Atlantic/Western Approaches

In each of the culture descriptions below, the first entry gives the culture identifier; the second entry gives details about when and by whom the culture was isolated; the third entry details when it was collected; the fourth entry details where it was collected; and the fifth entry notes whether or not the cells possess coccoliths.

92E; 29 June 92/J.C. Green; 24 June 92; 49,52N 6,12W; yes

92F; 29 June 92/J.C. Green; 24 June 92; 49,52N 6,12W; no

G1779 Ga; 11 Oct 89/J.C. Green; 14 June 89; 60,N 20,W; yes

G1779 Ge; 11 Oct 89/J.C. Green; 14 June 89; 60,N 20,W; no

5/90/25B; 25 Sept 90/J.C. Green; 25 May 90; 48,20N 17,33W; yes

5/90/25G; 25 Sept 90/J.C. Green; 25 May 90; 48,20N 17,33W; yes

5/90/25J; 25 Sept 90/J.C. Green; 25 May 90; 48,20N 17,33W; yes

5/90/25L; ; 25 Sept 90/J.C. Green; 25 May 90, 48,20N 17 ,3W; yes

DWN53/74/6; 8 Jan 91/J.C. Green; 8 Oct 90; 24,27N 20,24W; yes

DWN 61/3/2; 14 Sept 91/J.C. Green; 11 July 91; 58,48N 15,53W; yes

DWN 61/3/7; 24 Sept 91/J.C. Green; 11 July 91; 51,48N 15,53W; yes

DWN61/4/6; 3 Dec 91/J.C. Green; 11 July 91; 51,48N 15,53W; yes

DWN61/4/7; 3 Dec 91/J.C. Green; 11 July 91; 51,48N 15,53W; yes

DWN61/4/17; 24 Dec 91/J.C. Green; 11 July 91; 51,48N 15,53W; yes

DWN 61/6/2; 4 Dec 91/J.C. Green; 11 July 91; 51,48N 15,53W; yes

DWN 61/6/4; 4 Dec 91/J.C. Green; 11 July 91; 51,48N 15,53W; yes

DWN 61/6/5; 4 Dec 91/J.C. Green; 11 July 91; 51,48N 15,53W; yes

DWN 61/6/8; 4 Dec 91/J.C. Green; 11 July 91; 51,48N 15,53W; yes

DWN 61/6/10; 4 Dec 91/J.C. Green; 11 July 91; 51,48N 15,53W; yes

DWN 61/7/3; 29 Nov 91/J.C. Green; 11 July 91; 51,48N 15,53W; yes

DWN 61/7/4; 29 Nov 91/J.C. Green; 11 July 91; 51,48N 15,53W; yes

DWN 61/7/7; 29 Nov 91/J.C. Green; 11 July 91; 51,48N 15,53W; yes

DWN 61/7/9; 29 Nov 91/J.C. Green; 11 July 91; 51,48N 15,53W; yes

DWN 61/7/11; 29 Nov 91/J.C. Green; 11 July 91; 51,48N 15,53W; yes

DWN 61/12/2; 4 Dec 91/J.C. Green; 12 July 91; 61,05N 20W; yes

DWN 61/12/4; 4 Dec 91/J.C. Green; 12 July 91; 61,05N 20W; yes

DWN 61/12/5; 4 Dec 91/J.C. Green; 12 July 91; 61,05N 20W; no

DWN 61/12/6; 4 Dec 91/J.C. Green; 12 July 91; 61,05N 20W; yes

DWN 61/12/7; 4 Dec 91/J.C. Green; 12 July 91; 61,05N 20W; yes

DWN 61/12/9; 4 Dec 91/J.C. Green; 12 July 91; 61,05N 20W; yes

DWN 61/67/2; 29 Nov 91/J.C. Green; 23 July 91; 60,41N 20,16W; yes

DWN 61/67/3; 29 Nov 91/J.C. Green; 23 July 91; 60,41N 20,16W; yes

DWN 61/67/5; 29 Nov 91/J.C. Green; 23 July 91; 60,41N 20,16W; yes

DWN 61/67/9; 29 Nov 91/J.C. Green; 23 July 91; 60,41N 20,16W; yes

DWN 61/81/3; 29 Nov 91/J.C. Green; 26 July 91; 56,35N 19,58W; yes

DWN 61/81/5; 29 Nov 91/J.C. Green; 26 July 91; 56,35N 19,58W; yes

DWN 61/81/10; 29 Nov 91/J.C. Green; 26 July 91; 56,35N 19,58W; yes

DWN 61/81/12; 29 Nov 91/J.C. Green; 26 July 91; 56,35N 19,58W; yes

DWN 61/87/10; 17 Sept 91/J.C. Green; 26 July 91; 56 14N 19,58W; yes

DWN 61/87/17; 26 Sept 91/J.C. Green; 26 July 91; 60,33N 19,24W; yes

Clones Isolated During Bergen Mesocosm Experiments (1992/1993)

In each of the culture descriptions below, the first entry gives the culture number; the second entry gives the date when the culture was isolated; the third entry gives the bag number, or other location where the culture was collected (all in the Bergen area); and the fourth entry notes whether or not the cells possess coccoliths.

1. Numbers should all be prefixed 'B92' or 'B93' depending on the year of isolation.
2. Bag numbers: details of the mesocosm bags and their nutrient status can be found in [Egge JK and Heimdal BR (1994) "Blooms of phytoplankton including Emiliania huxleyi (Haptophyta). Effects of nutrient supply in different N:P ratios." Sarsia, 79: 333-348].
3. 'Fj' = Raunefjord water from outside the bags.
4. The position of the University of Bergen Marine Station is: 60,16N 5,14E.

8; 29 April 1992; 4; yes

9; 29 April 1992; 4; yes

11; 30 April 1992; 3; yes

13; 30 April 1992; 3; yes

12; 30 April 1992; 3; yes

15; 30 April 1992; 4; yes

18; 30 April 1992; 4;

20; 30 April 1992; Trengereid; yes

21; 30 April 1992; Trengereid; yes

22; 30 April 1992; Trengereid; yes

23; 30 April 1992; Trengereid; yes

24; 30 April 1992; Trengereid; yes

26; 30 April 1992; Trengereid; yes

27; 1 May 1992; 3; yes

28; 1 May 1992; 3; yes

29; 1 May 1992; 3; yes

31; 1 May 1992; 4; yes

32; 1 May 1992; 4; yes

38; 1 May 1992; Trengereid; yes

39; 1 May 1992; Trengereid; yes

43; 2 May 1992; 3; yes

46; 2 May 1992; 4; yes

49; 4 May 1992; Fj; yes

50; 4 May 1992; Fj; yes

52; 4 May 1992; Fj; yes

53; 4 May 1992; Fj; yes

54; 4 May 1992; Fj; yes

56; 4 May 1992; 5; yes

58; 4 May 1992; 5; yes

59; 4 May 1992; 5; yes

65; 4 May 1992 (Collected 3 May); 6; yes

69; 5 May 1992; 5; yes

70; 5 May 1992; 5; yes

72; 5 May 1992; Fj; yes

73; 5 May 1992; Fj; yes

75; 5 May 1992; Fj; yes

76; 5 May 1992; Fj; yes

78; 7 May 1992; 3; yes

81; 7 May 1992; Fj; yes

82; 7 May 1992; Fj; yes

84; 7 May 1992; 5; yes

86; 12 May 1992; 3; yes

91; 12 May 1992; Lygre; yes

92; 12 May 1992; Lygre; yes

95; 12 May 1992; Venjaneset (Fusafj.); yes

96; 12 May 1992; Venjaneset ; yes

97; 12 May 1992; Venjaneset ; yes

108; 13 May 1992; Fj.; yes

120; 13 May 1992; Samnangerfj.; yes

121; 13 May 1992; Samnangerfj.; yes

126; 14 May 1992; 6; yes

127; 14 May 1992; 6; yes

130; 14 May 1992; 6; yes

131; 14 May 1992; 3; yes

139; 15 May; Samnangerfj. (off Tysse); yes

145; 15 May; Samnangerfj. (off Tysse); yes

148; 15 May; Samnangerfj. (off Tysse); yes

149; 15 May; Samnangerfj. (off Tysse); yes

159; 15 May; Samnangerfj. (off Tysse); yes

163; 15 May; Samnangerfj. (off Tysse); yes

167; 15 May; Samnangerfj. (off Sandvik); yes

168; 15 May; Samnangerfj. (off Sandvik); yes

170; 16 May 1992; 3; yes

174; 16 May 1992; 6; yes

184; 19 May 1992; 6; yes

189; 19 May 1992; 6; yes

190; 19 May 1992; Vage; yes

202; 20 May 1992; 6; yes

204; 20 May 1992; 6; yes

208; 24 May 1992; 3; yes

211; 25 May 1992; 6; yes

212; 20 May 1992; 6; yes

214; 20 May 1992; 6; yes

217; 20 May 1992; 7; yes

220; 27 May 1992 (collected 26 May); Hardangerfj. (Sta. I: Gronnevikov 59,59.9N 5,48.2E); yes

221; 27 May (collected 26 May); Hardangerfj. (Sta. I); yes

222; 27 May (collected 26 May); Hardangerfj. (Sta. II, Kalven off Rosendal 59,59.4N 5,57.6E); yes

223; 27 May (collected 26 May); Hardangerfj. (Sta. II); yes

228; 27 May (collected 26 May); Hardangerfj. (Sta. IV, Onarheimsfj, 59,56.8N 5,39.5E) ; yes

229; 27 May (collected 26 May); Hardangerfj. (Sta. IV, Onarheimsfj.); yes

230; 29 May (Collected 28 May); Skjellanger, Holsnoy; yes

234; (Collected 30 May); Hardangerfj. (Head of Maurangerfj., nr. Sunndal) ; ?

317; 24 May; 3; yes

324; 25 May; 7; yes

328; 27 May (collected 26 May); Hardangerfj. (Sta. I); yes

329; 27 May (collected 26 May); Hardangerfj. (Sta. I); yes

330; 27 may (collected 26 May); Hardangerfj. (Sta. I); yes

331; 27 May (collected 26 May); Hardangerfj. (Sta. I); yes

333; 27 May (collected 26 May); Hardangerfj. (Sta. II); yes

336; 27 May (collected 26 May); Hardangerfj. (Sta. II); yes

340; 27 May (collected 26 May); Hardangerfj. (Sta. III, Storsundet/Vagsneset, 59,56.2N 5,40.0E) ; yes

345; 27 May (collected 26 May); Hardangerfj. (Sta. IV, Onarheimsfj.); yes

347; 27 May (collected 26 May); Hardangerfj. (Sta. IV, Onarheimsfj.); yes

349; 27 May (collected 26 May); Hardangerfj. (Sta. IV, Onarheimsfj.); yes

350; 27 May (collected 26 May); Hardangerfj. (Sta. IV, Onarheimsfj.); yes

354; 29 May (collected 28 May); Skjellanger, Holsnoy; yes

353; 29 May (collected 28 May); Skjellanger, Holsnoy; yes

356; 29 May (collected 28 May); Skjellanger, Holsnoy; yes

362; (Collected 30 May); Saevereidfj.; yes

364; (Collected 30 May); Saevereidfj.; yes

366; (Collected 30 May); Saevereidfj.; yes

367; (Collected 30 May); Saevereidfj.; yes

370; (Collected 30 May); Hardangerfj. (Head of Maurangerfj., nr. Sunndal); yes

371; ?; Hardangerfj. (Head of Maurangerfj., nr. Sunndal.); yes

378; (Collected 30 May); Eiklandsfj. (Eiklandsosen); yes

379; (Collected 30 May); Eiklandsfj. (Eiklandsosen); yes

386; (Collected 30 May); Samnangerfj. (Tysse); yes

400; 18 May, 93; Bag L1; yes

402; 18 May, 93; Bag L1; yes

403; 18 May, 93; Bag L1; yes

406; 18 May, 93; Bag H1; yes

407; 18 May, 93; Bag H1; yes

409; 18 May, 93; Bag H1; yes

410; 18 May, 93; Bag H1; yes

413; 19 May, 93; Fauskangerpollen, 10m (60,31N, 05,02E); yes

416; 19 May, 93; Fauskangerpollen, 3m; yes

417; 19 May, 93; Fauskangerpollen, 3m; yes

421; 19 May, 93; Fauskangerpollen, 3m; yes

422; 19 May, 93; Fauskangerpollen, 3m; yes

425; 19 May, 93; Fauskangerpollen, 3m; yes

426; 19 May, 93; Fauskangerpollen, 3m; yes

427; 19 May, 93; Fauskangerpollen, 3m; yes

428; 19 May, 93; Fauskangerpollen, 3m; yes

432; 20 May, 93; Raunefj.; yes

433, ; 20 May, 93; Raunefj.; yes

436; 20 May, 93; Raunefj.; yes

438; 20 May, 93; Raunefj.; yes

446; 21 May, 93; Mesocosm bag H1; yes

447; 21 May, 93; Mesocosm bag H1; yes

451; 21 May, 93; Mesocosm bag L2; yes

456; 21 May, 93; Mesocosm bag L2; yes

460; 21 May, 93; Raunefj.; yes

461; 21 May, 93; Raunefj.; yes

462; 21 May, 93; Raunefj.; yes

463; 21 May, 93; Raunefj.; yes

464; 21 May, 93; Raunefj.; yes

466; 21 May, 93; Raunefj.; yes

472; 22 May, 93; Mesocosm bag H1; yes; [Dilution culture]

473; 22 May, 93; Mesocosm bag H1; yes; [Dilution culture]

474, ; 22 May, 93; Mesocosm bag H1; yes; [Dilution culture]

476; 22 May, 93; Mesocosm bag H1; yes; [Dilution culture]

478,; 22 May, 93; Mesocosm bag H1; yes; [Dilution culture]

479, ; 22 May, 93; Mesocosm bag H1; yes; [Dilution culture]

481; 22 May, 93; Mesocosm bag H1; yes; [Dilution culture]

484; 22 May, 93; Mesocosm bag H1; yes; [Dilution culture]

487; 22 May, 93; Mesocosm bag H1; yes; [Dilution culture]

499; 22 May, 93; Mesocosm bag H1; yes; [Dilution culture]

500; 23 May, 93; Frommereid, 4m (60,31N 05,02E); yes

501; 23 May, 93; Frommereid, 4m (60,31N 05,02E); yes

502; 23 May, 93; Frommereid, 4m (60,31N 05,02E); yes

504; 23 May, 93; Frommereid, 4m (60,31N 05,02E); yes

506; 23 May, 93; Frommereid, 4m (60,31N 05,02E); yes

509; 23 May, 93; Frommereid, 4m (60,31N 05,02E); yes

518; 23 May, 93; Fauskangerpollen (60,31N 05,02E); yes

Miscellaneous

In each of the culture descriptions below, the first entry gives the culture designation; the second entry gives information about when the culture was isolated; the third entry gives the date of collection; the fourth entry says where the culture was collected from; and the fifth entry notes whether or not the cells possess coccoliths.

92A; Parke; July 1957; English Channel; no

92D; July 75; June 1975; English Channel (50,02N 04,22W); some

S. Africa; Pienaar; March 1983; West St.Pier, Durban; yes

Bgn June 91a; B.R. Heimdal; June, 1991; Bergen; yes?

Bgn June 91b; B.R. Heimdal; June, 1991; Bergen; yes?

CH24/90; NIOZ; June 1990; Rockall Bank; yes

CH25/90; NIOZ; June 1990; Rockall bank; yes

F61; From Paasche; ; Oslo Fjord; no

VAN55; NEPCC, UBC, Vancouver; March, 1976; 50,11N 144,57W; no

VAN556; NEPCC, UBC, Vancouver; June 1984; 49,5N 144,40W; no

Additional strains

1. A number of strains have recently been acquired from NIOZ, Texel, The Netherlands. These include a number of those listed above together with additional strains from the N. Atlantic and N. Sea.
2. Isolates from the Adriatic Sea, set up from material brought to Plymouth in 1993, are also available. However, precise details on the source of the original samples have not been obtainable.

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